96-Well Microplate & Pipetting Worklist
ELISA / qPCR Automated Assay Layout • ISO 17025 Cl. 7.2 • Hamilton / Tecan Worklist GeneratorMicroplate ID
PLT-96-20261113-7060
Standard 96-Well
DuplicateHorizontal
Well Occupancy
88 / 96 Wells
36 Samples •
12 Standards •
2 Blanks •
2 Controls
Pipetting Parameters
100 μL / well
Sample Dilution: 1:10 (10 μL sample + 90 μL buffer)
Assay Protocol
ISO 17025 Cl. 7.2 Immunoassay Protocol
Operator: Quality Analyst
2D Plate Map: ELISA Quantitative Antibody Titration Run #1
Blank (BLK)
Neg Control (NC)
Pos Control (PC)
Standard (S1-S6)
Sample (Duplicate)
1
2
3
4
5
6
7
8
9
10
11
12
A
B
C
D
E
F
G
H
Well Pipetting Inspector
Click any of the 96 wells on the plate map to view liquid handling volumes, dilution ratios, and specimen attribution.
Automated Pipetting Worklist (Tecan / Hamilton / Manual Execution)
160 Transfer Steps| Step # | Source Vessel | Destination | Liquid Component | Volume (μL) | Transfer Description |
|---|---|---|---|---|---|
| 1 | Diluent Buffer Reservoir (Trough 1) | A01 | Assay Buffer | 100.0 μL | Dispense buffer into A01 for Blank dilution (100 uL) |
| 2 | Diluent Buffer Reservoir (Trough 1) | A03 | Assay Buffer | 90.0 μL | Dispense buffer into A03 for UnknownSample dilution (90 uL) |
| 3 | Diluent Buffer Reservoir (Trough 1) | A04 | Assay Buffer | 90.0 μL | Dispense buffer into A04 for UnknownSample dilution (90 uL) |
| 4 | Diluent Buffer Reservoir (Trough 1) | A05 | Assay Buffer | 90.0 μL | Dispense buffer into A05 for UnknownSample dilution (90 uL) |
| 5 | Diluent Buffer Reservoir (Trough 1) | A06 | Assay Buffer | 90.0 μL | Dispense buffer into A06 for UnknownSample dilution (90 uL) |
| 6 | Diluent Buffer Reservoir (Trough 1) | A07 | Assay Buffer | 90.0 μL | Dispense buffer into A07 for UnknownSample dilution (90 uL) |
| 7 | Diluent Buffer Reservoir (Trough 1) | A08 | Assay Buffer | 90.0 μL | Dispense buffer into A08 for UnknownSample dilution (90 uL) |
| 8 | Diluent Buffer Reservoir (Trough 1) | A09 | Assay Buffer | 90.0 μL | Dispense buffer into A09 for UnknownSample dilution (90 uL) |
| 9 | Diluent Buffer Reservoir (Trough 1) | A10 | Assay Buffer | 90.0 μL | Dispense buffer into A10 for UnknownSample dilution (90 uL) |
| 10 | Diluent Buffer Reservoir (Trough 1) | A11 | Assay Buffer | 90.0 μL | Dispense buffer into A11 for UnknownSample dilution (90 uL) |
| 11 | Diluent Buffer Reservoir (Trough 1) | A12 | Assay Buffer | 90.0 μL | Dispense buffer into A12 for UnknownSample dilution (90 uL) |
| 12 | Diluent Buffer Reservoir (Trough 1) | B01 | Assay Buffer | 100.0 μL | Dispense buffer into B01 for Blank dilution (100 uL) |
| 13 | Diluent Buffer Reservoir (Trough 1) | B03 | Assay Buffer | 90.0 μL | Dispense buffer into B03 for UnknownSample dilution (90 uL) |
| 14 | Diluent Buffer Reservoir (Trough 1) | B04 | Assay Buffer | 90.0 μL | Dispense buffer into B04 for UnknownSample dilution (90 uL) |
| 15 | Diluent Buffer Reservoir (Trough 1) | B05 | Assay Buffer | 90.0 μL | Dispense buffer into B05 for UnknownSample dilution (90 uL) |
| 16 | Diluent Buffer Reservoir (Trough 1) | B06 | Assay Buffer | 90.0 μL | Dispense buffer into B06 for UnknownSample dilution (90 uL) |
| 17 | Diluent Buffer Reservoir (Trough 1) | B07 | Assay Buffer | 90.0 μL | Dispense buffer into B07 for UnknownSample dilution (90 uL) |
| 18 | Diluent Buffer Reservoir (Trough 1) | B08 | Assay Buffer | 90.0 μL | Dispense buffer into B08 for UnknownSample dilution (90 uL) |
| 19 | Diluent Buffer Reservoir (Trough 1) | B09 | Assay Buffer | 90.0 μL | Dispense buffer into B09 for UnknownSample dilution (90 uL) |
| 20 | Diluent Buffer Reservoir (Trough 1) | B10 | Assay Buffer | 90.0 μL | Dispense buffer into B10 for UnknownSample dilution (90 uL) |
| 21 | Diluent Buffer Reservoir (Trough 1) | B11 | Assay Buffer | 90.0 μL | Dispense buffer into B11 for UnknownSample dilution (90 uL) |
| 22 | Diluent Buffer Reservoir (Trough 1) | B12 | Assay Buffer | 90.0 μL | Dispense buffer into B12 for UnknownSample dilution (90 uL) |
| 23 | Diluent Buffer Reservoir (Trough 1) | C03 | Assay Buffer | 90.0 μL | Dispense buffer into C03 for UnknownSample dilution (90 uL) |
| 24 | Diluent Buffer Reservoir (Trough 1) | C04 | Assay Buffer | 90.0 μL | Dispense buffer into C04 for UnknownSample dilution (90 uL) |
| 25 | Diluent Buffer Reservoir (Trough 1) | C05 | Assay Buffer | 90.0 μL | Dispense buffer into C05 for UnknownSample dilution (90 uL) |
| 26 | Diluent Buffer Reservoir (Trough 1) | C06 | Assay Buffer | 90.0 μL | Dispense buffer into C06 for UnknownSample dilution (90 uL) |
| 27 | Diluent Buffer Reservoir (Trough 1) | C07 | Assay Buffer | 90.0 μL | Dispense buffer into C07 for UnknownSample dilution (90 uL) |
| 28 | Diluent Buffer Reservoir (Trough 1) | C08 | Assay Buffer | 90.0 μL | Dispense buffer into C08 for UnknownSample dilution (90 uL) |
| 29 | Diluent Buffer Reservoir (Trough 1) | C09 | Assay Buffer | 90.0 μL | Dispense buffer into C09 for UnknownSample dilution (90 uL) |
| 30 | Diluent Buffer Reservoir (Trough 1) | C10 | Assay Buffer | 90.0 μL | Dispense buffer into C10 for UnknownSample dilution (90 uL) |
| 31 | Diluent Buffer Reservoir (Trough 1) | C11 | Assay Buffer | 90.0 μL | Dispense buffer into C11 for UnknownSample dilution (90 uL) |
| 32 | Diluent Buffer Reservoir (Trough 1) | C12 | Assay Buffer | 90.0 μL | Dispense buffer into C12 for UnknownSample dilution (90 uL) |
| 33 | Diluent Buffer Reservoir (Trough 1) | D03 | Assay Buffer | 90.0 μL | Dispense buffer into D03 for UnknownSample dilution (90 uL) |
| 34 | Diluent Buffer Reservoir (Trough 1) | D04 | Assay Buffer | 90.0 μL | Dispense buffer into D04 for UnknownSample dilution (90 uL) |
| 35 | Diluent Buffer Reservoir (Trough 1) | D05 | Assay Buffer | 90.0 μL | Dispense buffer into D05 for UnknownSample dilution (90 uL) |
| 36 | Diluent Buffer Reservoir (Trough 1) | D06 | Assay Buffer | 90.0 μL | Dispense buffer into D06 for UnknownSample dilution (90 uL) |
| 37 | Diluent Buffer Reservoir (Trough 1) | D07 | Assay Buffer | 90.0 μL | Dispense buffer into D07 for UnknownSample dilution (90 uL) |
| 38 | Diluent Buffer Reservoir (Trough 1) | D08 | Assay Buffer | 90.0 μL | Dispense buffer into D08 for UnknownSample dilution (90 uL) |
| 39 | Diluent Buffer Reservoir (Trough 1) | D09 | Assay Buffer | 90.0 μL | Dispense buffer into D09 for UnknownSample dilution (90 uL) |
| 40 | Diluent Buffer Reservoir (Trough 1) | D10 | Assay Buffer | 90.0 μL | Dispense buffer into D10 for UnknownSample dilution (90 uL) |
| 41 | Diluent Buffer Reservoir (Trough 1) | D11 | Assay Buffer | 90.0 μL | Dispense buffer into D11 for UnknownSample dilution (90 uL) |
| 42 | Diluent Buffer Reservoir (Trough 1) | D12 | Assay Buffer | 90.0 μL | Dispense buffer into D12 for UnknownSample dilution (90 uL) |
| 43 | Diluent Buffer Reservoir (Trough 1) | E03 | Assay Buffer | 90.0 μL | Dispense buffer into E03 for UnknownSample dilution (90 uL) |
| 44 | Diluent Buffer Reservoir (Trough 1) | E04 | Assay Buffer | 90.0 μL | Dispense buffer into E04 for UnknownSample dilution (90 uL) |
| 45 | Diluent Buffer Reservoir (Trough 1) | E05 | Assay Buffer | 90.0 μL | Dispense buffer into E05 for UnknownSample dilution (90 uL) |
| 46 | Diluent Buffer Reservoir (Trough 1) | E06 | Assay Buffer | 90.0 μL | Dispense buffer into E06 for UnknownSample dilution (90 uL) |
| 47 | Diluent Buffer Reservoir (Trough 1) | E07 | Assay Buffer | 90.0 μL | Dispense buffer into E07 for UnknownSample dilution (90 uL) |
| 48 | Diluent Buffer Reservoir (Trough 1) | E08 | Assay Buffer | 90.0 μL | Dispense buffer into E08 for UnknownSample dilution (90 uL) |
| 49 | Diluent Buffer Reservoir (Trough 1) | E09 | Assay Buffer | 90.0 μL | Dispense buffer into E09 for UnknownSample dilution (90 uL) |
| 50 | Diluent Buffer Reservoir (Trough 1) | E10 | Assay Buffer | 90.0 μL | Dispense buffer into E10 for UnknownSample dilution (90 uL) |
Showing first 50 of 160 pipetting steps. Use "Export Worklist CSV" for complete liquid handler payload.